1. Place 1/4 seed into each well of a 96-round well deep well plate.
2. Add 400ul SDS extraction buffer (200 mM Tris-HCl pH 7.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS) to each well. Place in 70°C incubator for 2 hours, shaking at 225 rpm. Preheat shaker/incubator to 80°C.
3. Add 50ul 5 M NaCl to each well. Pipette samples 4 times to mix. Incubate at 4°C for 5'. Centrifuge for 5' at 3600 rpm.
4. Add 2/3 volume isopropanol (130ul) into a new 96-deep well plate.
5. Aspirate off 200ul supernatant and add to the isopropanol plate. Mix. Allow to sit 10' at RT.
6. Centrifuge for 15' at 3600 rpm. Carefully decant isopropanol (handle gently, pellets can be slippery).
7. Add 200ul 70% ethanol. Centrifuge 5' at 3600 rpm.
8. Carefully decant ethanol and allow to dry at 80°C in shaker/incubator until residual alcohol has evaporated (30-60'). Using high heat for this step seems to help remove proteins and other debris that can inhibit PCR.
9. Resuspend in 20 - 50ul DI water or TE (10 mM Tris-HCl pH 8, 1 mM EDTA). Store at 4°C overnight and gently vortex to mix. Quantify a few samples to estimate DNA concentration range across the plate and dilute to 20-50 ng/ul.
10. Centrifuge and transfer samples to a 96-well PCR plate. Use top portion for PCR.

Reference
Edwards, et al. Nucleic Acids Research 19(6): 1349.