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Outputs (last update = 6 October 2007)
Protocols & Tools
Promoter Discovery
Transgenics
A key role of the Center and its participants has been to develop robust and efficient systems for genetic engineering and regeneration of soybean from tissue culture. Here are the protocols we have developed:
Promoters are necessary to control the expression of engineered genes. Accordingly, center members have tested various promoters to determine their eventual usefulness to develop improved soybean varieties. Lately, microarray expression data from soybean somatic embryos has been used to isolate new promoters which will be useful to engineer improved seed composition traits in soybean.
GFP shows where lectin is expressed in a soybean embryo
GUS-staining reveals expression of glutamine synthetase in the seed coat
GUS-staining reveals expression of glutamine synthetase in the anthers
Leaf-vein-specific expression of the 35S promoter
GFP reveals expression of the 35S promoter in cultured embryogenic tissue
GFP reveals expression of the Gmubi promoter in cultured embryogenic tissue
GFP reveals expression of the HSP90 promoter in cultured embryogenic tissue
Actin and Gmubi root-specific promoters
Finally, Center members have evaluated several different genes to determine their potential for use in development of better soybean varieties. Here are some examples:
Soybean expressing a Bt gene for insect resistance
Soybean plants resistant to Bean Pod Mottle Virus coat protein
Soybean plants engineered to express oxalate oxidase for fungal resistance
Soybean expression a methionine-rich 15kD zein gene from corn, for better amino acid balance
Resistance to soybean mosaic virus
Specialty carotenoids for animal feed