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Soybean Somatic Embryogenesis and Transformation
Effect of light on embryogenesis |
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The top row of each cultivar represents cotyledons exposed to 50-60 µE m2 s-1, while those in the lower row of each cultivar were exposed to 5-10 µE m2 s-1. Photos were taken 30 d after explanting. The light was provided by cool-white fluorescent tubes. Results may differ if different a different type of light is used. All photos are at the same magnification:
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cv. Jack
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cv. Fayette
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For more information on light effects, see:
J. P. Ranch, L. Oglesby, and A. C. Zielinski. 1986. Plant regeneration from tissue cultures of soybean by somatic embryogenesis. In: Cell culture and somatic cell genetics of plants, edited by I. K. Vasil, New York:Academic Press. p. 97-110. P. A. Lazzeri, D. F. Hildebrand, and G. B. Collins. 1987. Soybean somatic embryogenesis: effects of nutritional, physical and chemical factors. Plant Mol. Biol. Rep. 10:209-220. |
