Soybean Somatic Embryogenesis and Transformation
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Literature
Literature

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Soybean Somatic Embryogenesis and Transformation

Effect of glutamine

Adding glutamine and other amino acids to liquid medium during the histodifferentiation and maturation phase leads to larger embryos which reach physiological maturity in about 5 weeks, but which germinate more quickly and vigorously. The best combination to date appears to be 30 mM glutamine and 1 mM of methionine:

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Effect of 0, 15, 30 60, and 120 mM of glutamine on embryo growth

References:

Schmidt, M.A., D.M. Tucker, E.B. Cahoon, and W.A. Parrott. 2005. Towards normalization of soybean somatic embryo maturation. Plant Cell Rep. 24:383-391.

  • Use of methionine:

  • Dyer DJ, Cotterman CD, Cotterman JC (1987) Comparison of in situ and in vitro regulation of soybean seed growth and development. Plant Physiol 84:298-303.

  • Use of glutamine:

  • Skokut TA, Varner JE, Schaefer J, Stejskal EO, McKay RA (1982) [15N] NMR determination of asparagine and glutamine nitrogen utilization for synthesis of storage protein in developing cotyledons of soybean in culture. Plant Physiol 69:308-313.