Soybean Somatic Embryogenesis and Transformation
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Soybean Somatic Embryogenesis and Transformation

Freezing for long-term storage of soybean seeds

Posted = 6 October 2007

Overall comments:

Soybean seed can be frozen for long-term storage. We have successfully frozen and germinated seed for over 15 years, with no measurable loss of viability, at both -20 and -80ºC. The procedure is very simple, using small Tupperware containers that contain:

freezer-box.gif - 19313 Bytes

  • A layer of Drier-rite on the bottom
  • A layer of seeds on top
  • A piece of filter paper to separate the two
After placing the seeds in the container and closing the lid, they are allowed to equilibrate for a few days at room temperature before placing in the freezer.